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The clinical laboratory department practice in hospital is very important in the teaching process of medical laboratory science and medical laboratory technology science .It is the transition link of guiding the students to go to the clinical job .This paper analyzes the problems and challenges in the process of clinical teaching and management of the trainees majored in medical laboratory science and medical laboratory technology science .Then summarizes the countermeasures to effectively cultivate these trainees , including setting up a teaching group , doing the overall quality control for the process of practice teaching using ISO15189 quality control methods for reference , establishing the evaluation mechanism for both trainees and tutors , founding virtual laboratory to improve the teaching effect of morphology , strengthening the consciousness of quality control , expanding the professional vision of interns and strengthening the cultivation of the scientific research ability of interns .
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Objective To investigate the levels of platelet-derived growth factor-BB (PDGF-BB) and its role in the pathogenesis of the patients with hepatic diseases.Methods The levels of serum PDGF-BB in 30 chronic patients (hepatitis B,26; hepatitis C,4),24 patients with post-hepatitis cirrhosis,30 patients with primary hepatocellular carcinoma,and 20 normal controls were measured by ELISA.The levels of PDGF-B mRNA in peripheral blood mononuclear cells were measured by reverse transcription polymerase chain reaction (RT-PCR).Results The serum levels of PDGF-BB and the levels of PDGF-B mRNA in peripheral blood mononuclear cells were significantly higher in the patients with hepatic diseases (F=1774.40,1037.42,P
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Objective:To analyses distribution and resistant profile of acquired infections of acinetobacter baumanii.Methods:Distribution and the resistant patterns of acquired infections of acinetobacter baumanii in our hospital were analysed by a retrospective analysis from the year 2003~2005.All of the isolates were tested by Kirby-Bauer susceptibility,and the antibiotic resistance was judged according to NCCLS standard.Results:The isolation rate of Acinetobacter baumannii was the higher in vena pipe,sputum,secretion and bile,the isolation rate was 11.5%~37.5%respectively.Primary acquired infections were Acinetobacter baumannii in department of breathe,burn,neurosurgery,heart internal medicine,transplantation,chest surgery and intensive care units.The bacterial resis-tancet rate to common antimicrobial agents increased year by year.There sistance rate s of Acinetobacter baumannii to antibiotics such as Cefop-erazone,Ampicillin,Ceftriaxone,Cefuroxime,Amoxicillin/Clavulanic Acid were over 50 % every year respectively.Levofloxacin,Meraopenem,Imipenem,Cefopera-zone/Sulbactam,Ampicillin/Sulbactam and Piperacillin/Tazobactam all had antisepsis activity to Acinetobacter baumannii relatively.Conclusion:The infection rate and the antibiotics rate of hospital acquired infections of Acinetobacter baumannii was yearly increase,resistance to anti-microbial agents have the characteristics of high prevalence.Its necessary to strengthen the clinic monitoring and select reasonable antibiotic in order to improve curative effect and delay bacteria of resistance to antimicrobial agents.
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Objective To investigate the distribution and drug resistance of pathogens of ventilator-associated pneumonia(VAP) in general intensive care unit(ICU).Methods Statistical methods were used to analyze retrospectively the data of pathogens and drug resistance of VAP in general ICU from 2004 to 2006.Results Totally 347 pathogens were isolated from deep-part secretion of lower respiratory tracts of 302 VAP in general ICU.The main pathogens included Pseudomonas aeruginosa(32.3%),Acinetobacter baumanii(31.1%),Staphylococcus aurous(16.7%),Klebsiella(7.5%) and Coagulase-negative Staphylococci(6.1%).Gram-negative bacillus showed a high resistance to piperacillin and Cefotaxime but high susceptibility to carbapenems and the enzyme inhibitor antibiotics.Gram-positive coccobacteria showed a high resistance to Penicillin,Clindamycin and Erythromycin but high susceptibility to Vancomycin and Quinolone.Conclusion Gram-negative bacillus was the main pathogen of VAP in general ICU,multidrug resistance was serious.The rate of methicillin-resistant staphylococcus aurous(MRSA) and methicillin-resistant coagulase-negative staphylococci(MRCNS) was high,with serious drug resistance.According to the data of the bacteria pathogen and antibiotics susceptibity,we can select antibiotics reasonably to control the infection and delay the emergency of new drug-resistant bacteria.